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Tuesday, April 21, 2020 | History

2 edition of Mutants of Chinese hamster cells in vitro found in the catalog.

Mutants of Chinese hamster cells in vitro

Michael William McBurney

Mutants of Chinese hamster cells in vitro

  • 223 Want to read
  • 37 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Cell metabolism,
  • Cell proliferation,
  • Hamsters

  • Edition Notes

    ContributionsToronto, Ont. University.
    The Physical Object
    Paginationvi, 188 leaves.
    Number of Pages188
    ID Numbers
    Open LibraryOL20175034M

    Apr 15,  · [Click the right of the frame to move the timeline or use the slider below] CHO recap. Since they were first isolated in the late s, Chinese hamster ovary (CHO) cells have become the expression system of choice for biomanufacturers with the firms behind top sellers like Rituxan, Humira and Enbrel all using them to manufacture their products. A collection of Chinese Hamster Ovary (CHO) Cell Culture Protocols for research, provided by Invitrogen. An "actual" Hamster Huey book was written by Mabel Barr in , years after. Janet Tashjian ( words) High frequency mutants of Chinese hamster ovary cells involve structural alterations. DIDS ( words is a potent inhibitor of DNA topoisomerase II in vitro and in Chinese hamster fibrosarcoma cells". Biochemical Pharmacology. 49 (3. Get this from a library! Molecular genetics of mammalian cells. Volume [Michael M Gottesman;] -- Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are.


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Mutants of Chinese hamster cells in vitro by Michael William McBurney Download PDF EPUB FB2

Srinivasan PR, Gupta RS, Siminovitch L. Studies on temperature-sensitive mutants of Chinese hamster ovary cells affected in DNA synthesis. Somatic Cell Genet. Sep; 6 (5)– Stanley P, Siminovitch L.

Selection and characterization of Chinese hamster ovary cells resistant to the cytotoxicity of lectins. In ggl14i.top by: 7. Temperature-sensitive clones have been selected from a mutagenized culture of Chinese hamster lung cells by a procedure involving bromodeoxy-uridine (BrdU) incorporation and irradiation with black light.

The selection procedure used in these studies was Cited by: Inaki Azpiazu, N. Gautam, in Methods in Enzymology, CHO Cell Culture and Membrane Purification. CHO (Chinese hamster ovary) cells expressing muscarinic receptors were developed by Dr. Peralta and have been successfully used in studies of G-protein and receptor interaction.

2,5,6 Cells are thawed from a liquid nitrogen frozen stock and seeded. May 17,  · Mammalian Cell Transfection and In vitro Mutagenesis. The Gene structure for adenosine kinase in Chinese hamster and human: high-frequency mutants of CHO cells involve deletions of several introns and exons.

X.A., Agarwal, T., Singh, B. et al. Molecular Characterization of Chinese Hamster Cells Mutants Affected in Adenosine Kinase and Cited by: 6. When V-B11 was fused with the mutants, many UV-resistant hybrids were formed in all cases, as is shown in Table 1.

The comple- mentation of V-B11 by all 6 mutants indicates that V-B11 represents a new, 7th complementation group of UV-sensitive mutants in Chinese hamster ggl14i.top by: @article{osti_, title = {Autoradiographic assay of mutants resistant to diphtheria toxin in mammalian cells in vitro}, author = {Ronen, A.

and Gingerich, J.D. and Duncan, A.M.V. and Heddle, J.A.}, abstractNote = {Diptheria toxin kills mammalian cells by ribosylating elongation factor 2, a protein factor necessary for protein synthesis.

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins.

@article{osti_, title = {The mutation studies of mutagen-sensitive and DNA repair mutants of Chinese hamster fibroblasts}, author = {Schultz, R.A. and Chang, C.C. and Trosko, J.E.}, abstractNote = {We have previously reported the isolation and partial characterization of DNA repair and/or mutagen-sensitive mutant Chinese hamster cell strains.

their synthesis. Mutants of mammalian cells affected in glyco-sylation pathways are a valuable resource for experiments aimed at structure/function analysis of mammalian glycans. A panel of glycosylation mutants that has been widely used for such purposes is the Chinese hamster ovary (CHO)4 mutant.

Aug 01,  · Abstract. Following chemical mutagenesis and multiple-step indirect selection, four clones of Chinese hamster V79 cells were isolated which exhibited auxotrophy for thymidine, deoxycytidine, or deoxyuridine but not for cytidine or ggl14i.top by: Jan 01,  · The responses of irs1 and irs2 have some similarities to those of cells from persons with the cancer-prone syndrome ataxia telangiectasia (AT), but the dissimilarities between both irs mutants and AT cell lines suggest that the primary defects are distinct.", T1 - Biochemical and genetic analysis of the Chinese hamster mutants irs1 and irs2 Cited by: Molecular Characterization of Chinese Hamster Cells Mutants Affected in Adenosine Kinase and Showing Novel Genetic and Biochemical Characteristics Xianying A Cui, Tanvi Agarwal, Bhag Singh and Radhey S Gupta* Abstract Background: Two isoforms of the enzyme adenosine kinase (AdK), which differ at their N-terminal ends, are found in mammalian ggl14i.top by: 6.

Molecular Characterization of Chinese Hamster Cells Mutants Affected in Adenosine Kinase and Showing Novel Genetic and Biochemical ggl14i.top R E S E A R C H A R T I C L E Open Access.

Genetic characterizations of the Chinese hamster ovary cell mutants resistant to the DNA polymerase inhibitors (aphidicolin, ara-A and ara-C) have been described.

Resistance to all three inhibitors showed dominance among the progeny of somatic cell crosses between the wild type and mutant ggl14i.top by: 4. Feb 19,  · Glycomics Profiling of Chinese Hamster Ovary Cell Glycosylation Mutants Reveals N-Glycans of a Novel Size and Complexity* Chinese hamster ovary (CHO) glycosylation mutants isolated on the basis of their lectin resistance have been particularly useful for glycosylation engineering of recombinant glycoproteins.

Mutants of mammalian cells. Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA ggl14i.top is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune ggl14i.tops: XRCC5, KARP-1, KARP1, KU80, KUB2.

Folate Deficient Mutants of Chinese Hamster Cells Characterization of a Chinese Hamster Cell with a Temperature-Sensitive Mutation in Folate Metabolism The Degradation of Ribonucleic Acids Injected into Xenopus laevis Oocytes Poly Adenylic Acid Synthesis In Vitro in Isolated.

hamster cells. For example, two of the major applications ox~ molecular cell genetics are the production of monodonal anti- bodies and the use of terato- carcinomas to study early embry- onic development.

Neither has been studied in hamster cells. An honest title for this book. The cross-resistances of several mutants of Chinese hamster ovary cells which have been obtained after one and two selection steps in the presence of the microtubule inhibitor podophyllotoxin Author: Radhey Gupta.

Chinese hamster ovary cell mutants defective in the NPC1 gene (NPC1-trap) were generated by retrovirus-mediated gene trap mutagenesis from a parental cell line JP17 expressing an ecotropic retrovirus receptor. Insertion of the gene trap vector in the NPC1 gene and the absence of the gene product were verified by 5'RACE and immunological analyses, ggl14i.top by: Jul 01,  · Abstract.

Positive selection-negative selection gene targeting was used to disrupt the nucleotide excision repair gene ERCC1 in a Chinese hamster ovary cell line, CHO-K1. Southern and Northern analysis showed that a cell clone isolated by this targeting approach, CHO, had an ERCC1 gene structure consistent with targeted disruption of ERCC1 exon V, and did not express ERCC1 ggl14i.top by: Sep 21,  · In addition, we compare the in vitro sensitivities of representative mutant PDGFRA isoforms to the kinase inhibitor imatinib in transiently transfected Chinese hamster ovary cells and stably transfected BA/F3 cells.

Our findings are summarized together with other recent reports of PDGFRA mutations to provide an overview of this subset of ggl14i.top by: the life of CHO cells in serum-free batch culture. Also, through our experiments we sought to better understand the pathway of apoptosis in CHO cells.

MATERIALS AND METHODS Cell Culture Chinese Hamster Ovary (CHO) cells expressing g-inter-feron were obtained from the Laboratory of Molecular Bi-ology, State University of Ghent, Belgium (Scahill. Unfortunately, this book can't be printed from the OpenBook. If you need to print pages from this book, we recommend downloading it as a PDF.

Visit ggl14i.top to get more information about this book, to buy it in print, or to download it as a free PDF. Aug 04,  · T he Chinese hamster has led a secret life in science for decades.

By one estimate from the Chemical Heritage Foundation, 11 biotech drugs that are made using the ovary cells of these small. today are made in Chinese hamster ovary (CHO) cells (Jayapal et al.

The popularity of CHO cells can be attributed to the following reasons. Firstly, since CHO cells have been dem-onstrated as safe hosts for the past two decades, it may be easier to obtain approval to market the therapeutic proteins from regulatory agencies like the FDA.

Mammalian cell lines are important host cells for the industrial production of pharmaceutical proteins owing to their capacity for correct folding, assembly and post-translational modification. In particular, Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins.

havior of karyoplasts andcytoplasts derived froma Chinese hamster ovary (CHO) cell line. These observations extend thoseinearlierreports (8,9) andestablisha basic background in the experimental use of karyoplasts andcytoplasts for the analysis of nuclear-cytoplasmic interactions.

MATERIALSANDMETHODS Chinese hamster ovary cells were grown to. December 21, Subject: Chinese Hamster Ovary Cell Specimens. Background: It has come to our attention that there is a question of whether or not Chinese hamster (Cricetulus griseus) ovary (CHO) cell specimens require a U.S.

Fish and Wildlife Service (FWS) declaration and clearance. Hamsters are considered wildlife and are not a. Sep 22,  · Effects of sunitinib and imatinib on autophosphorylation of (A) wild-type KIT and KIT mutants transiently expressed in Chinese hamster ovary cells; (B) KIT mutants expressed by gastrointestinal stromal tumor cell lines; or (C) platelet-derived growth factor receptor α mutants transiently expressed in Chinese hamster ovary ggl14i.top by: thermotolerance-deficient mutants of Chinese hamster ovary cells Callaghan, J.

M., Toh, B.H., Pettitt, J. M., Humphris, D. and Gleeson, P. Poly-iV-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. Identification of a tomato-lectin binding A/X 10 r membrane glycoprotein of tubulovesicles Methods in Cell Biology.

Academic Press, Jan 13, - Science - pages. 0 Reviews. Methods in Cell Biology. Preview this book. Furthermore, the in vitro biological activity of the mature BMP‐7 from PC5/6ΔC‐overexpressing cells was comparable to that from CHO‐BMP‐7 cells.

Taken together, the present results indicate that overexpression of PC5/6ΔC in CHO‐BMP‐7 cells is an efficient means of. Urlaub and L. Chasin, “Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity,” Proceedings of the National Academy of Sciences of the United States of America, vol.

77, no. 7, pp. –, View at: Google ScholarCited by: 6. Chinese hamster ovary (CHO) cells were introduced in the early s as a viable epithelial cell line containing twin female X chromosomes. The most common type of CHO cells has a nutritional requirement for the amino acid proline, which makes this cell line an ideal candidate for genetic studies.

human cells. Mutat Res *Ahmed FE, Lewis NJ, Hart RW. Pesticide induced ouabain resistant mutants in Chinese hamster V79 cells.

Chem Biol Interactions Ahmed NA, Rawi SM, el-Behary MH. Effect of dieldrin injection on the level of certain amino acids and some enzymes in rat brain. Comp Biochem Physiol Chinese hamster ovary cells / Michael M. Gottesman --Cultured S49 mouse T lymphoma cells / Theodoor van Daalen Peter C.

Brown --Replica plating and detection of antigen variants / Susannah Gal --Drug-resistant mutants: L. Scott Cram --Methods for chromosome banding of human and experimental tumors in Vitro / Jeffrey M. Trent.

Other in vitro mammalian gene mutation assays exist including those that use either Chinese hamster cell lines (CHO, AS52, and V79) or human lymphoblastoid cells (TK6). In these cell lines the. "Requirement for proliferating cell nuclear antigen expression during stages of the Chinese hamster ovary cell cycle." Biochemistry.

Apr 4; 28(7) 3/ Wilson KM, Keng PC. "Radiation-induced DNA damage and repair in quiescent and proliferating human tumor cells in vitro.".

Diphtheria toxin resistance in Chinese hamster cells: Genetic and biochemical characteristics of the mutants affected in protein synthesis.

Som. Cell Genet. 6: Srinivasan, P.R., Gupta, R.S. and Siminovitch, L. Studies on temperature sensitive mutants of Chinese hamster ovary cells affected in DNA synthesis. Som. CHO cells have a culture history of more than 50 years, since they were established from the ovary of a Chinese Hamster and became “immortal” in the laboratory of Dr.

Theodore Puck in the late s [2,3]. For the sake of simplicity, we call the first immortalized Cited by: Paclitaxel (Taxol®) is a member of the taxane class of anticancer drugs and one of the most common chemotherapeutic agents used against many forms of cancer.

Paclitaxel is a microtubule-stabilizer that selectively arrests cells in the G2/M phase of the cell cycle, and found to induce cytotoxicity in a time and concentration-dependent ggl14i.top by: Nov 22,  · Construction of BAC-based physical map for Chinese hamster ovary cells.

A CHO genomic BAC library consisted ofclones was constructed in a previous study [].Three hundred BAC clones were randomly selected from this library and mapped onto each chromosome of CHO DG44 cells by ggl14i.top by: 1.